Monochlorobimane
Monochlorodiamine (mBCl) is essentially non-fluorescent until it reacts with a thiol group. It readily reacts with several low molecular weight thiols,including cysteine,glutathione,N-acetylcysteine,mercaptopurine,peptides,and plasma thiols. The glutathione conjugate of mBCl has an absorption/emission maximum of ~394/490 nm.