Caspase-5 Activity Assay Kit

Determination of Significance：

Caspase is a family of proteases involved in the process of apoptosis, including more than 10 members. Caspase-2, also known as Ich-1 or Nedd-2, is activated in the process of apoptosis signal transduction. There are two different splicing variants of caspase-2 mRNA. The protein translated by the full-length mRNA can promote apoptosis, while the short protein product can inhibit apoptosis. Caspase 2 can be activated by caspase-1, caspase-3 and granzyme B.

Measurement Principle：

The caspase-2 colorimetric assay is based on the hydrolysis of the peptide substrate N-acetyl-Val-Asp-Val-Ala-Asp-pNA (Ac-VDVAD-pNA) by caspase-2, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. The activity of Caspase can be calculated by detecting pNA. This kit is suitable for mammalian tissue and cell.

Self Provided：

Spectrophotometer/Microplate reader, 100μL cuvette/ 96-well plate, centrifuge, water bath / incubator, adjustable pipette, mortar / homogenizer, ice and distilled water

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