Caspase-1 Activity Assay Kit

Determination of Significance：

α-L-rhamnosidase α(EC 3.2.1.40) catalyzes the hydrolysis of flavonoid glycosides and terpenes such as rutin, hesperidin and naringin at the end of α-L-rhamnosidase. The optimal pH for most α-L-rhamnosidases is in the acidic or near-neutral range. Fungal α-L-rhamnosidases mostly prefer a more acidic environment, with an optimal pH range of 4.0–6.5. On the other hand, bacterial-derived α-L-rhamnosidase mostly prefers a near-neutral or alkaline environment, with an optimal pH range of 5.0–8.0. This kit is suitable for the determination of α-L-rhamnosidase activity in acidic environments.

Measurement Principle：

Under acidic conditions, α-L-rhamnosidase and p-nitrophenol-α-rhamnoside (PNPR) produce yellow p-nitrophenol (PNP), which has a maximum absorption peak at 400 nm. By measuring the rate of increase of PNP at 400 nm, the magnitude of acidic α-L-rhamnosidase activity can be obtained.

Self Provided：

Visible spectrophotometer, benchtop centrifuge, water bath, adjustable pipette, 1 mL glass cuvette, mortar/homogenizer/cell sonicator, ice, and distilled water.

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