Cotinine [BSA]

SPECIFICATION SHEET Rev 110727DAGHML009 Cotinine, BSA Conjugate Antigen (Cotinine) Cat. No. DAG401 Lot. No. (See product label) PRODUCT INFORMATION BACKGROUND Product Overview: Cotinine Bovine Serum Albumin Introduction: Cotinine is an alkaloid found in to Conjugate bacco and is also a metabolite of nicotine. The word Form: BSA, Liquid "cotinine" is an anagram of "nicotine". Cotinine is Applications: Suitable for use in ELISA. Each labora used as a biomarker for exposure to tobacco smoke tory should determine an optimum working titer for use and has also been sold as an antidepressant under in its particular application. Other applications have not the brand name Scotine. Similarly to nicotine, co been tested but use in such assays should not neces tinine binds to, activates, and desensitizes neuronal sarily be excluded. nicotinic acetylcholine receptors, though at much Warning: This product contains sodium azide, which lower potency in comparison. It has demonstrated has been classified as Xn (Harmful), in European Di nootropic and antipsychoticlike effects in scientific rective 67/548/EEC in the concentration range of 0.1– research. 1.0%. When disposing of this reagent through lead or Keywords: S()1Methyl5(pyridin3yl)pyrrolidin2 copper plumbing, flush with copious volumes of water one; 1Methyl5(3pyridinyl)2pyrrolidinone; Co to prevent azide buildup in drains. tinine; (5S)1Methyl5(3pyridinyl)pyrrolidin2one; 1 methyl5pyridin3ylpyrrolidin2one; (s)2 pyrrolidinon; (s)1methyl5(3pyridinyl)2 PACKAGING pyrrolidinone; S()1METHYL5(3PYRIDYL)2 Concentration: 8.9mg/ml PYRROLIDONE; (S)1Methyl5(3pyridyl)2 Buffer: 15mM Potassium phosphate, pH 7.2 contain pyrrolidinone, S()1Methyl5(3pyridyl)2 ing 0.85% Sodium chloride pyrrolidone Preservative: 0.1% Sodium azide Storage: aliquot and store at 20°C. Avoid multiple REFERENCES freeze/thaw cycles. 1. David J. Triggle (1996). Dictionary of Pharmacol ogical Agents. Boca Raton: Chapman & Hall/ CRC. ISBN 0412466309. 2. Dwoskin LP, Teng L, Buxton ST, Crooks PA (March 1999). "(S)()Cotinine, the major brain metabolite of nicotine, stimulates nicotinic recep tors to evoke [3Hdopamine release from rat stri atal slices in a calciumdependent manner"]. The Journal of Pharmacology and Experimental Therapeutics 288 (3): 905–11. PMID 10027825. 3. Anderson DJ, Arneric SP (March 1994). "Nicotinic receptor binding of [3Hcytisine, [3H] nicotine and [3H]methylcarbamylcholine in rat brain"]. European Journal of Pharmacology 253 (3): 261–7. doi:10.1016/00142999(94)902003. PMID 8200419. Creative Diagnostics. All rights reserved. 4516 Ramsey Road Shirley, NY 11967, USA Tel: 6316244882 ·Fax:6316147828 Email: info@creativediagnostics.com www.creativediagnostics.com