HBV Surface Antigen (subtype adw, mutation K-141-E)

RecombinantHepatitis B Surface Antigen (adw Mutant K141E) DAG3934 HBV Lot. No. (See product label) PRODUCT INFORMATION Product overview RecombinantHepatitis B Surface Antigen (adw Mutant K141E) Antigen Description HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection. The viral envelope of enveloped virus has different surface proteins from the rest of the virus which act as antigens. These antigens are recognized b Description Recombinant HBsAg adw antigen has a single mutation, lysine residue at position 141 replaced with glutamate. Source Pichia pastoris Species HBV Form Sterilefiltered solution containing 20 mM Na2HPO4, 0.03 M NaCl and 0.001% Thimerosal, pH 7.4. Purity Greater than 85% as determined by SDSPAGE Applications immunogen PACKAGING Storage Store at 4°C. BACKGROUND Introduction Hepatitis B is one of a few known nonretroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially doublestranded, relaxed circular genomes (RCDNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and subgenomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reversetranscribed into new RCDNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA. Keywords HBsAg protein; HBsAg protein; HBV major surface antigen protein; HBV surface antigen protein; S protein; S protein; HBsAg; HBsAg preS2 REFERENCES 1. Kimura T, Rokuhara A, Matsumoto A, et al. (May 2003). "New enzyme immunoassay for detection of hepatitis B virus core antigen (HBcAg) and relation between levels of HBcAg and HBV DNA". J. Clin. Microbiol. 41 (5): 1901–6. doi:10.1128/JCM.41.5.19011906.2003. PMC 154683. PMID 12734224. 2. Cao T, Meuleman P, Desombere I, Sällberg M, LerouxRoels G (December 2001). "In vivo inhibition of antihepatitis B virus core antigen (HBcAg) immunoglobulin G production by HBcAgspecific CD4(+) Th1type Tcell clones in a huPBLNOD/SCID mouse model". J. Virol. 75 (23): 11449–56. doi:10.1128/JVI.75.23.1144911456.2001. PMC 114731. PMID 11689626. Creative Diagnostics. All rights reserved 4516 Ramsey Road Shirley, NY 11967, USA Tel: 6316244882 ·Fax:6316147828 Email: info@creativediagnostics.com www.creativediagnostics.com