HIV type 1 Gag [His]

Recombinant Human Immunodeficiency Virus Type 1 Gag(Clade A), His tagged DAG2005 HIV Lot. No. (See product label) PRODUCT INFORMATION Product overview Cterminal 6xHis tagged HIV1 Gag(Clade A) protein (GenebankNo. AY772985). Antigen Description The Gag protein is the major structural protein required for virus assembly. It is synthesized as a polyprotein in the cytosol of an infected cell, and contains four functional segments; Matrix (MA), Capsid (CA), Nucleocapsid (NC), and p6. The NC region is flanked by two "spacer" segments, denoted SP1 and SP2. The polyprotein is all alpha helical, except the NC region, which is composed of two RNA interacting zinc knuckle domains. Gag is often referred to as an "assembly machine" because expression of Gag alone is sufficient to produce budding viruslike particles (VLP's), due to multimerization of roughly 2000 Gag molecules per virion. Gag is cleaved by the protease at multiple sites. The GAG proteins play important roles throughout the viral lifecycle, including the assembly and release of viral particles, their subsequent maturation into infectious virions, and during the events occurring between the release of capsids into newly infected cells and the integration of proviral DNA. During the early steps of the viral life cycle, viral proteins, especially capsid (CA), are in intimate contact with the intracellular environment. Considerable evidence supports the idea that interactions between host cellular proteins and the viral capsid are important for events occurring early in infection, such as the transport of the preintegration complex, uncoating of the capsid, nuclear entry, and integration Gag capsid (CA) protein can markedly reduce viral fitness, and interactions of CA with host proteins such as cyclophilin A (CypA) and TRIM5alpha can have important effects on viral infectivity. Source E. coli Species HIV Tag His Form Each vial contains 100 μg of lyophilized protein in500mM NaCl, 50mM Phosphate buffer (pH 7.4), 200mM Imidazole, 8M urea. Purity >= 90% Applications WB standard, antibody ELISA, etc PACKAGING Storage Store at 4˚C; DO NOT FREEZE; stable for 1 year from the date of shipment. Nonhazardous. No MSDS required Concentration 1 mg/ml Dilutions 100 μl Ultrapure water. BACKGROUND Introduction Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (AIDS), a condition in humans in which progressive failure of the immune system allows lifethreatening opportunistic infections and cancers to thrive. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, preejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. The four major routes of transmission are unsafe sex, contaminated needles, breast milk, and transmission from an infected mother to her baby at birth (perinatal transmission). Screening of blood products for