HCV type 1a Core Antigen (aa 2 - 119)

Recombinant Hepatitis C Virus Core(Genotype1a) DAG1986 Hepatitis C Virus Lot. No. (See product label) PRODUCT INFORMATION Product overview The E.coli derived recombinant protein contains the HCV core nucleocapsid immunodominant regions, amino acids 2119. Antigen Description The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of coremediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCVinfected patients and inhibit host immunity through an interaction with gC1qR. Source E. coli Species Hepatitis C Virus Tag N/A Form Each vial contains 100 µg of lyophilized protein in 50mM 50mM TrisHCl, pH8, 60mM NaCl, 10mM glutathione, 0.25% sarkosil & 50% glycerol. AA Sequence a.a. 2119 Purity >95% , based on SDS PAGE Applications WB standard, antibody ELISA, immunogen, etc. PACKAGING Storage Before reconstitution, stable for 1 year at 20˚C from the date of shipment. After reconstitution, stable for a month at 4˚C. Nonhazardous. No MSDS required Concentration N/A Dilutions with 100 µl of Millipore water. BACKGROUND Introduction Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10, 000 nucleotides and encodes a single polyprotein of about 3, 000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. Keywords HCcAg; Core protein p19; HCV core antigen; HCV core protein; Hepatitis C Virus core protein; HCV1 Core Ag; Hepatitis C Virus Core Antige, genotype 1a; Flaviviridae; Hepacivirus REFERENCES 1. Tellinghuisen TL, Paulson MS, Rice CM. The NS5A protein of bovine viral diarrhea virus contains an essential zincbinding site similar to that of the hepatitis C virus NS5A protein. J Virol. Aug 2006; 80(15):74508. Creative Diagnostics. All rights reserved 4516 Ramsey Road Shirley, NY 11967, USA Tel: 6316244882 ·Fax:6316147828 Email: info@creativediagnostics.com www.creativediagnostics.com Creative Diagnostics. All rights reserved 4516 Ramsey Road Shirley, NY 11967, USA Tel: 6316244882 ·Fax:6316147828 Email: info@creativediagnostics.com www.creatived