HSV type 1

SPECIFICATION SHEET Rev 111113AGHJW0003 Inactived Herpes Simplex Virus1 Antigen Antigen (HSV1) Cat. No: DAG179 Lot.No: (See product label) PRODUCT INFORMATION BACKGROUND Product Overview: Inactived Herpes Simplex Virus Introduction: Herpes simplex virus 1 and 2 (HSV1 1 Antigen is inactivated by heating to 56oC for 2 and HSV2), also known as Human herpes virus 1 hours and by the addition of 0.01% Triton X100. and 2 (HHV1 and 2), are two members of the her Inactivity is confirmed by double blind passage. pes virus family, Herpesviridae, that infect humans. Antigen Description: This product is a cell lysate Both HSV1 (which produces most cold sores) and that is useful for a range of techniques, but contains HSV2 (which produces most genital herpes) are soluble cytoplasmic proteins from the production ubiquitous and contagious. They can be spread cells. Herpes simplex virus 1 (HSV1) is a member of when an infected person is producing and shedding the Herpesviridae viral family. the virus. Symptoms of herpes simplex virus infec Product Form: Clarified crude lysate containing tion include watery blisters in the skin or mucous HSV1 particles and proteins liquid. membranes of the mouth, lips or genitals. Lesions Target Species: Viral heal with a scab characteristic of herpetic disease. Format: Inactivated Pathogen Sometimes, the viruses cause very mild or atypical Activity: This product has been rendered inactive by symptoms during outbreaks. However, as neuro standard procedures. However this material should tropic and neuroinvasive viruses, HSV1 and 2 per still be handled as infectious and should be disposed sist in the body by becoming latent and hiding from of appropriately the immune system in the cell bodies of nerves. After Preparation: HSV1 is propagated in mammalian the initial or primary infection, some infected people cells using a protocol optimised for maximum parti experience sporadic episodes of viral reactivation or cle yield. At the appropriate time, infected cells are outbreaks. In an outbreak, the virus in a nerve cell washed in saline and disrupted to release virus parti becomes active and is transported via the nerve's cles by freeze thaw and sonication. Virus particles axon to the skin, where virus replication and shed released from cells are partially purified by low ding occur and cause new sores. speed centrifugation to remove cell debris. The prod Keywords: HSV1; HSV; HHV; HHV1; Herpes sim uct is then inactivated by heating to 56oC for 2 hours plex virus 1; Herpes Simplex Virus Type 1; Herpes and by the addition of 0.01% Triton X100. Inactivity viridae; Alphaherpesvirinae; Simplexvirus is confirmed by double blind passage. Applications: ELISA PACKAGING REFERENCES Concentration: 0.7 mg/ml as determined by BCA assay. 1. Gupta R, Warren T, Wald A (2007). "Genital her Buffer Solution: Phosphate buffered saline 0.01% pes". Lancet 370(9605): 2127–37. Triton X100 ettenleiter TC, Klupp BG, Granzow H (2006).