YTHDF2 antibody (pAb), sample

YTHDF2 (YT521-B homology domain family 2, also known as CAHL, HGRG8, NY-REN-2) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF proteins contain a highly conserved single-stranded RNA-binding domain which can specifically recognize and bind m6A-containing RNAs. An important role of YTHDF2is in regulating mRNA stability. YTHDF2 recognizes many m6A-containing mRNAs that are not being actively translated and recruits them to cytoplasmic processing (P) bodies for degradation. YTHDF2 preserves 5'UTR methylation of stress- induced transcripts and promotes cap-independent mRNA translation by competitive binding and limiting the m6A 'eraser' FTO from demethylation. N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).