SET8 / PR-SET7 antibody (pAb), sample

SET8 (SET-domain containing protein 8, PR-SET7, SETD8, KMT5A) is a SET domain-containing histone methyltransferase that specifically monomethylates histone H4 Lys20. H4 Lys20 monomethylation is enriched during mitosis and is associated with transcriptional repression. SET8 mainly functions in euchromatic regions, playing a role in gene silencing. SET8 knockout is homozyhgous lethal in mice and its function is required for cell cycle progression and cell proliferation. SET8 is required for proper chromosome condensation during interphase and chromosome segregation during mitosis. SET8 is also involved in DNA damage repair, as cells lacking SET8 function accumulate massive amounts of DNA damage. SET8 has been found to methylate p53 at Lys382, indicating a potential mechanism for SET8s role in the DNA damage repair pathway.