Histone Modification ELISAs provide a simple, sensitive method for detecting changes in specific histone modifications from purified core histones or histones isolated by acid extraction. These kits are sandwich ELISAs that utilize a capture antibody against histone H3 and a primary antibody specifc for the modification of interest. A secondary antibody conjugated to horseradish peroxidase (HRP) and developing solutions provide a sensitive colorimetric readout that is easily quantified by spectrophotometry. The assay is performed in a convenient 96-stripwell plate, enabling low or high throughput screening. For complete details, click the ELISA Method tab below.
Histone H3 phospho Ser28 (H3S28ph) ELISA
Histone H3 phospho Ser28 ELISA (H3S28ph) kits provide everything need to screen for phosphorylated serine 28 on histone H3 in human, mouse, hamster and bovine systems. Histone H3 phosphorylation on serine 28 (H3 pSer28) has been correlated with chromosome condensation, making the phosphorylation state of Ser28 an interesting marker for cells undergoing mitosis. The phosphorylation specific histone H3 antibody will not cross-react with phosphorylation of serine 10. To determine histone H3 serine 10 phosphorylation levels, we also offer a Histone H3 phospho Ser10 ELISA. For added convenience, the Histone phosphorylation ELISAs include paclitaxel treated and untreated acid extract for use as positive and negative controls, respectively. Click the pS28 Info tab below for data and more information; kit manuals can be downloaded under the Documents tab.