GoTaq G2 Flexi DNA Polymerase (100u)

The second generation of GoTaq® products, GoTaq® G2 DNA Polymerase reliably amplifies a wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistency. The product is available in many formats to give you maximum flexibility, control and convenience for your PCR. For robust, routine PCR choose a standalone enzyme and buffer system with or without magnesium. For maximum convenience choose an all-in-one master mix.

For superior convenience with hot-start PCR see GoTaq® G2 Hot Start Polymerase.

GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Both buffers contain MgCl₂ at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction.

GoTaq® G2 Flexi DNA Polymerase is supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl₂.

GoTaq® G2 Green and Colorless Master Mixes are ready-to-use, green and colorless, 2X master mixes containing all necessary components for robust, reliable PCR, including the second-generation GoTaq® G2 DNA Polymerase. Add template, primers and go.

The GoTaq® G2 and G2 Flexi DNA Polymerases are supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´→3´ exonuclease activity. The 5X Green GoTaq® Reaction and Flexi Buffers contain two dyes (blue and yellow) that separate during electrophoresis to indicate migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.

Features - Benefits

Robust, Reliable Amplification

• Direct-to-gel amplification buffer.
• Two buffer systems available to match your needs:
  • Reaction buffer with MgCl₂ to simplify reaction setup.
  • Flexi buffer and separate MgCl₂ to enable optimization.
• Risk-Free: Backed by the Promega PCR Satisfaction Guarantee.

Guarantee

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.

Applications

• Complex genomic templates.
• T-vector cloning.
• RT-PCR.
• Two-step RT-PCR.
• Low-copy target.