GoTaq G2 Hot Start Colorless Master Mix (100 reactions)

GoTaq® G2 Polymerase is the second generation of GoTaq® products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience.

For superior convenience and improved yield, sensitivity and specificity, choose GoTaq® G2 Hot Start Polymerase, which is bound to a proprietary antibody that blocks activity. Activity is restored during initial denaturation, allowing hot-start PCR. Available as a master mix or standalone enzyme.

GoTaq® G2 Hot Start Polymerase is supplied with 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl₂. The high-performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94–95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C for convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´ exonuclease activity.

The GoTaq® G2 Hot Start Master Mixes are ready-to-use mixes containing all necessary components (GoTaq® G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium)—you only need to add primer and template and go!

The GoTaq® G2 Hot Start Green Master Mix also contains a gel loading dye to facilitate downstream gel analysis. The GoTaq® G2 Hot Start Colorless Master Mix contains no gel loading dye for use when downstream applications require fluorescence or absorbance readings without purification.

Features - Benefits

Robust reliable PCR for all your amplification needs.

• Simplify reaction setup and save time with a ready-to-use master mix.
• Prepare your reaction at room temperature, not on ice.
• Eliminate nonspecific amplification with hot-start enzyme.
• Use at no risk—backed by the Promega PCR Satisfaction Guarantee.

Guarantee

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.

Applications

• Hot-start PCR.
• Complex genomic templates.
• T-vector cloning.
• RT-PCR.
• Two-step RT-PCR.
• Low-copy target.