The GoTaq® Probe qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format. The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. This master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.
The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.
Features - Benefits
- Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
- Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
- Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons.
- Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.
Guarantee
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
Applications
- Gene expression analysis.
- Multiplex PCR.
- Genotyping.
- Detection of sequence variants.
Notes
| |
|
Number of Reactions |
| Product Cat.# |
Size |
50µl reactions |
20µl reactions |
10µl reactions |
| A6101 |
2ml |
80 reactions |
200 reactions |
400 reactions |
| A6102 |
10ml |
400 reactions |
1,000 reactions |
2,000 reactions |
| A6110 |
2ml |
50 × 20µl RT reactions and
80 × 50µl qPCR reactions |
50 × 20µl RT reactions and
200 × 20µl qPCR reactions |
50 × 20µl RT reactions and
400 × 10µl qPCR reactions |
| A6120 |
2ml |
80 reactions |
200 reactions |
400 reactions |
The GoTaq® Probe qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format. The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. This master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.
The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.
Features - Benefits
- Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
- Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
- Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons.
- Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.
Guarantee
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
Applications
- Gene expression analysis.
- Multiplex PCR.
- Genotyping.
- Detection of sequence variants.
Notes
| |
|
Number of Reactions |
| Product Cat.# |
Size |
50µl reactions |
20µl reactions |
10µl reactions |
| A6101 |
2ml |
80 reactions |
200 reactions |
400 reactions |
| A6102 |
10ml |
400 reactions |
1,000 reactions |
2,000 reactions |
| A6110 |
2ml |
50 × 20µl RT reactions and
80 × 50µl qPCR reactions |
50 × 20µl RT reactions and
200 × 20µl qPCR reactions |
50 × 20µl RT reactions and
400 × 10µl qPCR reactions |
| A6120 |
2ml |
80 reactions |
200 reactions |
400 reactions |
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