GoTaq qPCR Master Mix (1,000 x 50µl reactions)

The GoTaq® qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays using a double-stranded DNA-binding dye. The master mix contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum PCR efficiency and greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I.

The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

Features - Benefits

• Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
• Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
• Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments.
• Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.

Guarantee

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.

Applications

• Gene expression analysis.
• Genotyping.
• Detection of sequence variants.
• Sensitive sequence detection and quantification.

Notes