LigaFast Rapid DNA Ligation System (150 reactions)

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg²⁺ addition prior to use.

Features - Benefits

• Flexible: Use with 5´, 3´ or blunt-ended DNA inserts.
• Fast: Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature.
• Convenient: No requirement to purify ligated DNA prior to heat-shock transformation in E. coli. Ligations conducted at room temperature.
• Ready-To-Use: No additional buffer modifications required prior to use.
• Efficient: Ligations performed using the LigaFast™ System are comparable to standard overnight ligations.
• Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.

Applications

For more information, see the Protocols & Applications Guide.