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S1 Nuclease (10,000u)

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M5761

Description

S1 Nuclease degrades single-stranded DNA and RNA endonucleolytically to yield 5´-phosphoryl-terminated products. Double-stranded nucleic acids (DNA:DNA, DNA:RNA or RNA:RNA) are resistant to degradation except with extremely high concentrations of enzyme. The enzyme is used to remove single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts.

Features - Benefits

  • Provided with 10X Reaction Buffer: 0.5M sodium acetate (pH 4.5 at 25°C), 2.8M NaCl, 45mM ZnSO4.

Applications

  • Removal of protruding single-stranded termini in double-stranded DNA.
  • Selective cleavage of single-stranded DNA.
  • Mapping RNA transcripts.

References

  1. Vogt, V.M. (1973) Eur. J. Biochem. 33, 192–200.
  2. Roberts, T.M. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 760–4.
  3. Berk, A.J. and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75, 1274–8.

Specifications

QC Tests

Activity, unidirectional deletions using the Erase-a-Base™ System.

Source

Fungal α amylase powder.

Storage Buffer

20mM Tris-HCl (pH 7.5 at 25°C), 0.1mM ZnCl2, 50mM NaCl and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1μg of acid-soluble material per minute at 37°C in 30mM sodium acetate (pH 4.6 at 25°C), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5% glycerol.

For product intended use please see Patents & Disclaimers tab.

Protocols and manuals

Resources

Use Restrictions

M5761 For Research Use Only. Not for Use in Diagnostic Procedures.

Communication is Key 

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