M-MLV Reverse Transcriptase, RNase H Minus (10,000u)

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). This form of M-MLV Reverse Transcriptase has been genetically altered to remove the associated RNase H activity. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare long cDNAs and libraries containing a high percentage of full-length cDNA.

Features - Benefits

• RNase H Minus: Provides optimal conditions to prepare full-length cDNA from long RNA templates.
• Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl₂, 50mM DTT.
• May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.

Applications

• First-strand cDNA synthesis.
• Primer extension.

References

1. Roth, M.J. et al. (1985) J. Biol. Chem. 260, 9326–35.
2. Tanese, N. and Goff, S.P. (1988) Proc. Natl. Acad. Sci. USA 85, 1777–81.