Mung Bean Nuclease (2,000u)

Mung Bean Nuclease catalyzes the degradation of single-stranded DNA and RNA endonucleolytically to yield 5´-phosphoryl-terminated products. While the nuclease prefers ssDNA over dsDNA by 30,000-fold, at very high concentrations the enzyme degrades double-stranded DNA from both ends. Mung Bean Nuclease has been used for transcript mapping studies, for flushing staggered ends and for the separation of cDNA strands after synthesis with reverse transcriptase and DNA Polymerase I.

Features - Benefits

• Provided with 10X Reaction Buffer: 300mM sodium acetate (pH 5.0 at 15°C), 500mM NaCl, 10mM ZnCl₂.

Applications

• Removal of protruding single-stranded termini in double-stranded DNA.
• Selective cleavage of single-stranded nucleic acid, e.g., mRNA mapping experiments.

References

1. Ardelt, W. and Laskowski, M., Sr. (1971) Biochem. Biophys. Res. Comm. 44, 1205–11.
2. Kroeker, W.D. et al. (1976) Biochemistry 15, 4463–7.
3. Mathis, D.J. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 7383–7.
4. Green, M.R. and Roeder, R.G. (1980) Cell 22, 231–42.
5. Gubler, U. (1987) Meth. Enzymol. 152, 330–5.