RNase ONE Ribonuclease (5,000u)

RNase ONE™ Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3´-NMPs. RNase ONE™ Ribonuclease is one of the few known RNases that can cleave a phosphodiester bond between any two ribonucleotides. RNase ONE™ Ribonuclease may be used to remove RNA from DNA preparations, for RNase protection assays and for mapping or quantitation of RNA by selective cleavage of single-stranded regions.

Features - Benefits

• Flexible: RNase ONE™ Ribonuclease has the ability to cleave phosphodiester bonds between any two ribonucleotides.
• Provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 50mM EDTA, 2M sodium acetate.

Applications

• Mapping or quantitation of RNA by selective cleavage of single-stranded regions.
• RNase protection assays.
• Mismatch detection.
• Selective removal of RNA from DNA preparations.

For more information, see the Protocols & Applications Guide.

References

1. Shen, V. and Schlessinger, D. (1982) The Enzymes, Vol. XV, 501.
2. Meador, J., 3rd et al. (1990) Eur. J. Biochem. 187, 549–53.
3. Meador, J., 3rd and Kennell, D. (1990) Gene 95, 1–7.