Terminal Transferase Buffer Pack (3 x 500microl)

Terminal Deoxynucleotidyl Transferase, Recombinant, catalyzes the repetitive addition of mononucleotides to the terminal 3´-OH of a DNA initiator accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM Co²⁺ (as CoCl₂) in the reaction buffer allows the tailing of 3´-ends with varying degrees of efficiency.

Features - Benefits

• Tails Any Type of 3´ End: The presence of 1mM CoCl₂ in the reaction buffer allows the tailing of any type of 3´ end (3´ and 5´ overhangs or blunt ends).
• Tested for Apoptotic DNA Labeling: Each lot of enzyme is qualified for success in the procedure outlined in the DeadEnd™ Fluorometric TUNEL System Technical Bulletin #TB235.
• Provided with 5X Reaction Buffer: 500mM cacodylate buffer (pH 6.8 at 25°C), 5mM CoCl₂, 0.5mM DTT.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Applications

• Tailing reactions to add complementary homopolymer tails to DNA vectors and cDNA.
• 3´ end-labeling.
• TUNEL assays.

For more information, see the Protocols & Applications Guide.