GoScript Reverse Transcriptase (100 reactions)

GoScript™ Reverse Transcriptase utilizes the M-MLV reverse transcriptase enzyme and state-of-the-art buffer to drive robust, reliable cDNA synthesis of a full range of rare and abundant transcripts over a wide range of RNA input amounts (5µg–1pg). Optimized for use in qPCR, including with GoTaq® Real-Time PCR Systems, GoScript™ Reverse Transcriptase can handle the most challenging templates and is resistant to PCR inhibitors.

A Complete System
GoScript™ Reverse Transcription System is provided in a kit format that includes GoScript™ Reverse Transcriptase, reaction buffer and an optimized set of reagents, including MgCl₂, dNTP Mix, Oligo(dT), Random Primers and Recombinant RNasin® Ribonuclease Inhibitor, designed for efficient synthesis of first-strand cDNA. The components of the system can effectively reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA.

Mix with Oligo(dT)
GoScript™ Reverse Transcription Mix, Oligo(dT), is a convenient mix that includes the GoScript™ Enzyme Mix (GoScript™ Reverse Transcriptase and RNasin® Plus RNase Inhibitor) and the GoScript™ Reaction Buffer (Oligo(dT), MgCl₂ and dNTPs), which have been optimized to provide efficient synthesis of first-strand cDNA in preparation for qPCR amplification. The components of the GoScript™ Reverse Transcription Mix can be used to reverse transcribe total RNA, poly(A)+ mRNA or synthetic transcript RNA. The population of cDNAs produced using this method allows many different downstream analyses from a single reverse transcription reaction.

Mix with Random Primers
GoScript™ Reverse Transcription Mix, Random Primers, is provided as a user-friendly mix including the GoScript™ Enzyme Mix (GoScript™ Reverse Transcriptase and RNasin® Plus RNase Inhibitor) and the GoScript™ Reaction Buffer (Random Primers, MgCl₂ and dNTPs), which have been optimized to provide efficient synthesis of first-strand cDNA in preparation for qPCR amplification. Priming using random primers is the most general method of initiating cDNA synthesis from a variety of RNA templates. Random primers can be used to prime first-strand cDNA synthesis from all RNA molecules, including those that do not possess a poly(A)+ tail and RNA isolated from prokaryotic sources.

Stand-Alone Enzyme
GoScript™ Reverse Transcriptase contains the GoScript™ M-MLV reverse transcriptase enzyme and the 5X GoScript™ Reaction Buffer.

Features - Benefits

• Sensitive: Confident transcription of both high-copy and low-copy messages.
• Resilient: Synthesizes cDNA in the presence of strong PCR inhibitors.
• Processive: Transcribes short and long transcripts.
• Consistent: Processes through secondary structure.
• Flexible: Available as a component of master mixes, as part of a complete system or as a stand-alone enzyme for easy setup of optimized reactions.

Applications

• Reverse transcription. 
• RT-PCR and RT-qPCR.
• Incorporation of marked nucleotides.
• Primer extension/RACE.

Notes

GoScript™ Reverse Transcription System includes: GoScript™ Reverse Transcriptase, GoScript™ 5X Reaction Buffer, MgCl₂, PCR Nucleotide Mix, Oligo(dT)₁₅ Primer, Random Primers, Nuclease-Free Water and Recombinant RNasin® Ribonuclease Inhibitor.

GoScript™ Reverse Transcriptase includes GoScript™ Reverse Transcriptase, GoScript™ 5X Reaction Buffer and MgCl₂.

GoScript™ Reverse Transcription Mix, Oligo(dT), includes: GoScript™ Enzyme Mix (GoScript™ Reverse Transcriptase and RNasin® Plus RNase Inhibitor), GoScript™ Reaction Buffer, Oligo(dT) (dNTPs, MgCl₂ and Oligo (dT)₁₅ Primer) and Nuclease-Free Water.

GoScript™ Reverse Transcription Mix, Random Primers, includes GoScript™ Enzyme Mix (GoScript™ Reverse Transcriptase and RNasin® Plus RNase Inhibitor) and GoScript™ Reaction Buffer, Random Primers (dNTPs, MgCl₂ and Random Primer) and Nuclease-Free Water.