ImProm-II Reverse Transcriptase (10 reactions)

ImProm-II™ Reverse Transcriptase enables robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The ImProm-II™ Reverse Transcriptase can be used to reverse transcribe total RNA, poly(A)+ mRNA or synthetic transcript RNA templates.

Features - Benefits

• Full-Length RT-PCR: Reverse transcribe RNA templates up to 8.9kb.
• Microarray-Compatible: Incorporate regular and Cy®3- and Cy®5-modified nucleotides.
• Scalable and Flexible: Reaction volumes of 1–20μl can be used in subsequent PCR, and the optimized buffer also allows for coupled RT-PCR.
• Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl₂ Solution also is included.
• Versatile: Use with your thermostable polymerase of choice for two-step RT-PCR.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

References

1. Miller, K. (2001) Promega Notes 79, 15–17.