The DNA 5´ End-Labeling System is a complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 Polynucleotide Kinase and [γ-32P]ATP. The system includes enzymes, buffers and control DNA standards to measure reaction efficiencies. Calf Intestinal Alkaline Phosphatase is included for removal of the 5´-phosphate prior to labeling with T4 Polynucleotide Kinase.
Features - Benefits
- Convenient: Can use to label both single-stranded and double-stranded DNA and RNA.
- Complete: System includes enzymes, buffers and control DNA standards for measuring reaction efficiencies (except radionucleotides).
- Flexible: Works with [γ-32P]ATP, [γ-33P]ATP or [γ-35S]ATP.
Applications
- Preparation of hybridization probes.
- Preparation of sequencing primers.
- Labeling marker DNAs and RNAs for autoradiography.
- Preparation of probes for gel shift assays.
For more information, see the Protocols & Applications Guide.
The DNA 5´ End-Labeling System is a complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 Polynucleotide Kinase and [γ-32P]ATP. The system includes enzymes, buffers and control DNA standards to measure reaction efficiencies. Calf Intestinal Alkaline Phosphatase is included for removal of the 5´-phosphate prior to labeling with T4 Polynucleotide Kinase.
Features - Benefits
- Convenient: Can use to label both single-stranded and double-stranded DNA and RNA.
- Complete: System includes enzymes, buffers and control DNA standards for measuring reaction efficiencies (except radionucleotides).
- Flexible: Works with [γ-32P]ATP, [γ-33P]ATP or [γ-35S]ATP.
Applications
- Preparation of hybridization probes.
- Preparation of sequencing primers.
- Labeling marker DNAs and RNAs for autoradiography.
- Preparation of probes for gel shift assays.
For more information, see the Protocols & Applications Guide.
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