High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5.
dUTP (2´-Deoxyuridine, 5´-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is function-tested to ensure specific DNA amplification and the absence of nuclease activity.
Features - Benefits
- Dependable: PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzymes.
- Consistent: dUTP is ≥99% triphosphate, allowing highly consistent results.
- Convenient: Supplied at a convenient concentration (100mM in water) for ease-of-use in PCR and other applications.
Guarantee
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
Applications
For more information, see the Protocols & Applications Guide.
References
- Longo, M.C. et al. (1990) Gene 93, 125–8.
- Erlich, H.A. et al. (1991) Science 252, 1643–51.
High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5.
dUTP (2´-Deoxyuridine, 5´-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is function-tested to ensure specific DNA amplification and the absence of nuclease activity.
Features - Benefits
- Dependable: PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzymes.
- Consistent: dUTP is ≥99% triphosphate, allowing highly consistent results.
- Convenient: Supplied at a convenient concentration (100mM in water) for ease-of-use in PCR and other applications.
Guarantee
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
Applications
For more information, see the Protocols & Applications Guide.
References
- Longo, M.C. et al. (1990) Gene 93, 125–8.
- Erlich, H.A. et al. (1991) Science 252, 1643–51.
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