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Histone H3S10ph rat antibody (mAb) (Clone 6G8B7) (100 µg)

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61623

Description

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is strongly phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells.

Specifications


Application Notes

Validated Applications:
ICC/IF: 0.5 - 2 µg/ml dilution
WB: 0.2 - 2 µg/ml dilution
ELISA: Individual optimization required.
Immunogen
This antibody was raised against a peptide corresponding to amino acids 1-19 including phospho-serine 10 of human Histone H3.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guaranteefor Histone H3S10ph antibody (mAb) (Clone 6G8B7)Guarantee
This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

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