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AgeI (100u)

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R7251

Description

ACCGG  T
T   GGCCA

Features - Benefits

  • Rapid Digest Capable: Capable of digesting DNA in 15 minutes or less.
  • GoTaq® Buffer Compatible: Active and capable of digestion directly in GoTaq® Green Master Mix.

Notes

Ends generated with AgeI can be directly ligated to ends generated using AccIII, NgoMIV or XmaI. The AgeI recognition site is not regenerated in the ligation product.

Specifications

Frequency of Cutting

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
13 5 0 0 0 0

Incubation Conditions

Buffer K. 37°C.

Percent Activity in 4-CORE® Buffer

A B C D K MULTI-CORE™
25–50% 25–50% 25–50% 50–75% 100% 100%

Source

Agrobacterium gelatinovorum.

Storage Buffer

10mM Tris-HCl (pH 7.4), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol, 0.5mg/ml BSA.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Protocols and manuals

Related citations

Resources

Use Restrictions

R7251 For Research Use Only. Not for Use in Diagnostic Procedures.

Communication is Key 

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Experienced Experts

Our team of highly qualified application scientists can provide you with ongoing support as and when needed.