BclI (1,000u)

T^▼GATC  A
A  CTAG_▲T

Features - Benefits

• Genome Qualified: Promega's Genome-Qualified restriction enzymes are assayed to ensure optimal performance in genomic analysis applications.

Notes

1. Sensitive to dam methylation.
2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
3. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
4. Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.