Frequency of Cutting
| λ |
Ad-2 |
ΦX174 |
pUC18 |
M13mp18 |
pBR322 |
| 13 |
10 |
0 |
0 |
0 |
0 |
Incubation Conditions
Buffer D. 60°C. (BstEII exhibits 25–50% activity at 37°C.)
Percent Activity in 4-CORE® Buffer
| A |
B |
C |
D |
MULTI-CORE™ |
| 25–50% |
50–75% |
50–75% |
100% |
100% |
Source
Bacillus stearothermophilus.
Storage Buffer
10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Storage Conditions
Store at –20°C.
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