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Flexi System, Entry/Transfer (5 entry and 20 transfer reactions)

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C8640

Description

The Flexi® Vector System is a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions without the need to resequence.

All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert.

Unlike site-specific recombination vector systems, the Flexi® Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of the protein of interest. In addition, the systems do not require an archival entry vector, and most applications allow direct entry into the vector suited to the experimental design.

C-terminal Flexi® Vectors allow expression of C-terminal-tagged proteins. While these vectors can act as acceptors of protein-coding regions flanked by SgfI and PmeI, they lack a PmeI site and contain a different blunt-ended site, EcoICRI. This joined sequence cannot be removed from the C-terminal Flexi® Vectors and transferred to other Flexi® Vectors.

For more information about the HaloTag® Flexi® Vectors, please visit the HaloTag® Technology Products page.

Features - Benefits

  • Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian or cell-free protein expression) and then transfer to others as required.
  • Time Savings: Efficient transfer allows direct use of recombinant clones, minimizing time wasted screening background colonies.
  • Enhanced Productivity: Adaptable to high-throughput formats for large screening projects.
  • Easy Access: No licensing fees or complicated transfer restrictions.

Applications

  • Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.

For more information, see the Protocols & Applications Guide.

Note: For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit: www.genecopoeia.com and www.kasuza.or.jp.kop/dsearch-e

Specifications

Storage Conditions

Cat.# C8640 is comprised of Cat.# C8641 and A9280. Store Cat.# C8641 at –20°C; store Cat.# A9280 at room temperature. Store Cat.# C8820 and C9320 at –20°C. Store enzyme blends at –20°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

C8640, C8820, C9320, G6050, R1851, R1852, R1901 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G6050 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

C8640, C8820, C9320, G6050, R1851, R1852, R1901 U.S. Pat. Nos. 8,293,503 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

C8640, C8820, C9320, G6050 Patent Pending.

G6050 U.S. Pat. Nos. 7,425,436, 7,935,803, 8,466,269, 8,742,086, 8,420,367 and 8,748,148 and other patents and patents pending.

C8640, C8820, C9320, G6050 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

C8640, C8820, C9320, G6050, R1851, R1852 Licensed under U.S. Pat. No. 5,945,288.

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