ADP-Glo Kinase Assay + LCK Kinase Enzyme System (1 each)

See All Kinase Enzyme Systems
Recombinant full-length human LCK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LCK (p56lck) is a member of the src family of nonreceptor tyrosine kinases. It was identified as a gene rearranged and overexpressed in the murine lymphoma LSTRA, most likely as a result of the insertion of Moloney murine leukemia virus DNA immediately adjacent to the gene. LCK behaves as a proto-oncogene and can lead to cell transformation upon activation. A number of human cancer cell lines show overexpression of LCK, pointing to a possible role for this kinase in the initiation and maintenance of the transformed state in human cancers.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: LCK, 10μg (Human, recombinant full-length). MW: ~84kDa.
• Substrate: Poly (4:1 Glu, Tyr) Peptide.
• Other: Reaction Buffer, DTT, MnCl₂.

LCK NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1. Fischer, S. et al. (1987) Biochem. Biophys. Res. Commun. 143(3), 819–26.
2. Veillette, A. et al. (1987) Oncogene Res. 1(4), 357–74.