ADP-Glo Kinase Assay + ITK Kinase Enzyme System (1 each)

See All Kinase Enzyme Systems
Recombinant human ITK (amino acids 352–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ITK is a member of the TEC family of nonreceptor tyrosine kinases. ITK is expressed in T-cells and is important for T-cell development and activation through the antigen receptor. ITK requires prior activation of Lck, Zap-70 and PI3-kinase for efficient activation and shares major substrates with both Lck and Zap-70. ITK knockout mice show multiple effects on T cell development, cytokine production and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization and regulation of the signaling events involved in cytoskeletal reorganization.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: ITK, 10μg (Human, recombinant; amino acids 352–end). MW: ~53kDa.
• Substrate: Poly (4:1 Glu, Tyr) Peptide.
• Other: Reaction Buffer, DTT, MnCl₂.

ITK NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1. August, A. et al. (2002) Int. J. Biochem. Cell Biol. 34(10), 1184–9.
2. Finkelstein, L.D. et al. (2004) Trends Cell Biol. 14(8), 443–51.