ADP-Glo Kinase Assay + GSK3 alpha Kinase Enzyme System (1 each)

See All Kinase Enzyme Systems
Recombinant full-length human GSK3α was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3α is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and transcription factors (e.g., JUN). GSK3α also plays a role in the WNT and PI3K signaling pathways. Alzheimer disease is associated with increased production and aggregation of amyloid-β-40 and -42 peptides into plaques. GSK3α is required for maximal production of the β-amyloid-40 and -42 peptides generated from the amyloid precursor protein by presenilin-dependent γ-secretase cleavage. In vitro, lithium, a GSK3α inhibitor, blocks the production of the β-amyloid peptides by interfering with the γ-secretase step.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: GSK3α, 10μg (Human, recombinant full-length). MW: ~81kDa.
• Substrate: GSK3 Substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE); derived from human muscle glycogen synthase 1 (amino acid 636–661).
• Other: Reaction Buffer, DTT.

GSK3α NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1. Ali, A. et al. (2001) Chem. Rev. 101, 2527–40.
2. Phiel, C.J. et al. (2003) Nature 423, 435–9.