EGF Receptor (10u)

Epidermal Growth Factor Receptor (EGF Receptor) is a cell- surface glycoprotein composed of a single polypeptide chain that binds the peptide Epidermal Growth Factor (EGF). The EGF Receptor consists of an extracellular ligand binding domain, a single transmembrane region and a cytoplasmic intrinsic tyrosine kinase domain. Upon ligand binding, the EGF Receptor autophosphorylates, activating the tyrosine kinase domain of the EGF Receptor.

EGF Receptor is immunopurified from the A431 cell line following a procedure detailed by Weber et al. The purified EGF Receptor does possess tyrosine kinase activity due to the bound EGF; however, the EGF Receptor has not been autophosphorylated.

References

1. Weber, W. et al. (1984) J. Biol. Chem. 259, 14631–6.