ADP-Glo Kinase Assay + CK1 alpha 1 Kinase Enzyme System (1 each)

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Full-length recombinant human CK1α1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1α1 is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes. CK1 can regulate Smo cell surface accumulation and activity in response to hedgehog. CK1 phosphorylates Smo at several sites, and phosphorylation-deficient forms of Smo fail to accumulate on the cell surface and are unable to transduce the hedgehog signal (1). CK1α1 dynamically associates with the CBM complex on T cell receptor engagement to participate in cytokine production and lymphocyte proliferation. CK1α1 can form complexes with MDM2, which then regulates the stability of p53 and E2F-1 transcription factors (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: CK1α1, 10μg (Human, recombinant full-length). MW: ~62kDa.
• Substrate: Casein, dephosphorylated; native protein purified from bovine milk.
• Other: Reaction Buffer, DTT.

CK1α1 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1. Jia, J. et al. (2004) Hedgehog signalling activity of Smoothened requires phosphorylation by protein kinase A and casein kinase I. Nature 432, 1045–50. 
2. Huart, A.S. et al. (2009) CK1alpha plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability. J Biol Chem. 284, 32384–94.