ADP-Glo Kinase Assay + CK1 epsilon Kinase Enzyme System (1 each)

See All Kinase Enzyme Systems
Full-length recombinant human CK1ε (CK1epsilon) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1ε is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes, including DNA replication and repair. CK1ε is a regulator of Yes-associated protein (YAP) transcription coactivator, which is a key regulator of organ size and a candidate human oncogene. CK1ε is activated by CCK2R, and this then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells (1). CKIε can phosphorylate topoisomerase (topo) IIalpha at serine-1106, and this regulates the enzyme activity and sensitivity to topo II-targeted drugs (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: CK1ε, 10μg (Human, recombinant full-length). MW: ~72kDa.
• Substrate: Casein, dephosphorylated; native protein purified from bovine milk.
• Other: Reaction Buffer, DTT.

CK1ε NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1. von Blume J. et al. (2007) Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2. EMBO J. 26, 4619–33. 
2. Grozav, A.G. et al. (2009) Casein kinase I delta/epsilon phosphorylates topoisomerase IIalpha at serine-1106 and modulates DNA cleavage activity. Nucleic Acids Res. 37, 382–92.