c-MER Kinase Enzyme System (10microg)

See All Kinase Enzyme Systems
Recombinant human c-MER (amino acids 578–872) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. c-MER is a tyrosine kinase and a member of the MER/AXL/TYRO3 receptor kinase family. The c-MER gene encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains and one tyrosine kinase domain. Functional knockout of c-MER in mice shows the mice to have macrophages deficient in the clearance of apoptotic thymocytes thereby demonstrating the critical role for c-MER in the engulfment and efficient clearance of apoptotic cells. Defects in c-MER have been associated with retinitis pigmentosa, a phenotype that can be reversed by replacement of the c-MER gene product.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: c-MER, 10μg (Human, recombinant; amino acids 578–872). MW: ~58kDa.
• Substrate: Poly (4:1 Glu, Tyr) Peptide.
• Other: Reaction Buffer, DTT, MnCl₂.

c-MER NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1. Graham, D.K. et al. (1994) Cell Growth Differ. 5, 647–57.
2. Gal, A. et al. (2000) Nature Genet. 26, 270–1.