Active Motif rounds up the latest news in the field of Epigenetics.
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Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins
Maintenance of pluripotency is regulated by a network of transcription factors coordinated by Oct4, Sox2, and Nanog (OSN), yet little is known about their interaction with chromatin-related proteins and cofactors. In this paper, the authors introduce a novel protocol combining ChIP with selective isolation of chromatin-associated proteins (SICAP) followed by mass spectrometry to identify chromatin-bound partners of a protein of interest. Using mouse embryonic stem cells (ESC) as a model, they identified over 400 proteins associated with OSN.
Chromatin Kinases Act on Transcription Factors and Histone Tails in Regulation of Inducible Transcription
In the current study, researchers from C. David Allis’s lab investigate the crosstalk between mitogen- and stress-activated protein kinases (MSKs) and the transcription activation of genes involved in the inflammatory response. Results indicate that up-regulation of induced genes in mouse macrophages stimulated with bacterial lipopolysaccharide is followed by enrichment of H3K28ph through activation of MSKs, and that H3K28ph promotes p300/CBP-dependent transcription in cell-free transcription assays. This work uncovers the mechanistic action of MSK-induced transcription through crosstalk with chromatin factors.
MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria
In this recent Cell publication, researchers from the Akhtar lab reveal that MOF is a dual-transcriptional regulator of nuclear and mitochondrial genomes connecting epigenetics and metabolism. Specifically, they found that Mof depletion in mice leads to severe myopathies and dysfunction of mitochondria. By investigating the mechanistic events underlying the dysfunction, the authors identified that Mof binds to mtDNA and regulates a series of respiratory genes. Moreover, members of the non specific lethal complex are located in mitochondria, influencing Mof localization and function.